Hotspot

rpl33-rps18

LSC · 77153-77571 · 419 bp

Back to Croton

Pi 0.0579
MarkerSeek score 77.70
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
419
variable_sites
56
pi
0.0579
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.82
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 ACATACAATTCACGGGGAGA GCAAACGCCTACGAAAAGAC 45.0 50.0 56.2 58.1 346-508 bp 1.0 77.5
rpl33-rps18_p2 ACATACAATTCACGGGGAGA CTACGAAAAGACCGCTTGGA 45.0 50.0 56.2 57.9 338-500 bp 1.0 77.3
rpl33-rps18_p3 ACATACAATTCACGGGGAGA TACGAAAAGACCGCTTGGAC 45.0 50.0 56.2 58.2 337-499 bp 1.0 76.2
rpl33-rps18_p4 ACATACAATTCACGGGGAGA TCAATTCGATCCCCCGATTG 45.0 50.0 56.2 57.7 374-536 bp 1.0 75.9
rpl33-rps18_p5 AACATACAATTCACGGGGAGA GCAAACGCCTACGAAAAGAC 42.9 50.0 57.0 58.1 347-509 bp 1.0 75.6