Hotspot

ndhC-trnV-UAC

LSC · 58969-59730 · 762 bp

Back to Croton

Pi 0.0726
MarkerSeek score 80.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
762
variable_sites
107
pi
0.0726
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.87
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAATCAGAAGCA AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.4 57.8 469-916 bp 1.0 52.0
ndhC-trnV-UAC_p2 TCATATTCGTAAATCAGAAGCA GAAGGTCTACGGTTCGAGTC 31.8 55.0 53.4 57.5 468-915 bp 1.0 52.0
ndhC-trnV-UAC_p3 ATCATATTCGTAAATCAGAAGCA AGAAGGTCTACGGTTCGAGT 30.4 50.0 54.0 57.8 470-917 bp 1.0 52.0
ndhC-trnV-UAC_p4 TCATATTCGTAAATCAGAAGCAT AGAAGGTCTACGGTTCGAGT 30.4 50.0 54.0 57.8 469-916 bp 1.0 52.0
ndhC-trnV-UAC_p5 TCATATTCGTAAATCAGAAGCA GTTCGAGTCCGTATAGCCCT 31.8 55.0 53.4 58.7 457-904 bp 1.0 52.0