Hotspot

trnG-GCC-trnfM-CAU

LSC · 44724-44980 · 257 bp

Back to Croton

Pi 0.0836
MarkerSeek score 77.70
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
257
variable_sites
36
pi
0.0836
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.79
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 280-371 bp 1.0 90.4
trnG-GCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.8 57.8 265-356 bp 1.0 88.8
trnG-GCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 264-355 bp 1.0 88.8
trnG-GCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG AGTTCTTCTCTTCATCGCGC 50.0 50.0 57.8 58.4 301-392 bp 1.0 87.7
trnG-GCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG GGGGTAGAGCAGTTTGGTAG 50.0 55.0 57.8 57.3 281-372 bp 1.0 85.1