Hotspot

trnH-GUG-psbA

LSC · 1894-2306 · 413 bp

Back to Croton

Pi 0.0628
MarkerSeek score 77.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
413
variable_sites
59
pi
0.0628
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.85
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT CTGTCGAAGCTCCATCTATCA 50.0 47.6 57.9 57.0 387-498 bp 1.0 74.9
trnH-GUG-psbA_p2 ACTGCCTTAATCCACTTGGC CTGTCGAAGCTCCATCTATCA 50.0 47.6 58.2 57.0 388-499 bp 1.0 74.7
trnH-GUG-psbA_p3 AATCCACTTGGCTACATCCG CTGTCGAAGCTCCATCTATCA 50.0 47.6 57.7 57.0 380-491 bp 1.0 73.4
trnH-GUG-psbA_p4 CTGCCTTAATCCACTTGGCT TGTCGAAGCTCCATCTATCA 50.0 45.0 57.9 55.8 386-497 bp 1.0 73.1
trnH-GUG-psbA_p5 ACTGCCTTAATCCACTTGGC TGTCGAAGCTCCATCTATCA 50.0 45.0 58.2 55.8 387-498 bp 1.0 72.9