Hotspot

rpl32-ndhF

SSC · 124326-124986 · 661 bp

Back to Crotalaria

Pi 0.0415

MarkerSeek score 75.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 661
variable_sites: 50
pi: 0.0415
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AAATAAAATGCCATGCCGCC	GTGGGAAAAAGTAGGAGCCC	45.0	55.0	58.0	58.2	1315-1358 bp	1.0	86.0
rpl32-ndhF_p2	AAATAAAATGCCATGCCGCC	TTTGCTGCAATAGGACGTGT	45.0	45.0	58.0	58.1	1741-1784 bp	1.0	85.0
rpl32-ndhF_p3	AAATAAAATGCCATGCCGCC	TCCCGAAATTCGAAACTCCC	45.0	50.0	58.0	57.9	1843-1886 bp	1.0	84.8
rpl32-ndhF_p4	TCCTAAGAGCAGCGTGTCTA	TCCCGAAATTCGAAACTCCC	50.0	50.0	57.9	57.9	1789-1832 bp	1.0	84.4
rpl32-ndhF_p5	AAATAAAATGCCATGCCGCC	CCCGAAATTCGAAACTCCCA	45.0	50.0	58.0	58.2	1874 bp	0.25	48.1