Hotspot

ndhG-ndhE

SSC · 119763-119978 · 216 bp

Back to Crotalaria

Pi 0.044

MarkerSeek score 72.40

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 216
variable_sites: 16
pi: 0.044
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	GTGCAATTGTTGTGGCTCG	TTTCGGCTCGTGATCAATCC	52.6	50.0	58.2	58.3	319-346 bp	1.0	73.1
ndhG-ndhE_p2	GGTGCAATTGTTGTGGCTC	TTTCGGCTCGTGATCAATCC	52.6	50.0	57.8	58.3	320-347 bp	1.0	73.0
ndhG-ndhE_p3	GGTGCAATTGTTGTGGCTC	CGGCTCGTGATCAATCCATA	52.6	50.0	57.8	57.6	317-344 bp	1.0	72.4
ndhG-ndhE_p4	GTGCAATTGTTGTGGCTCG	CGGCTCGTGATCAATCCATA	52.6	50.0	58.2	57.6	316-343 bp	1.0	72.4
ndhG-ndhE_p5	GGTGCAATTGTTGTGGCTC	AACCATATTTCGGCTCGTGA	52.6	45.0	57.8	57.3	327-354 bp	1.0	70.3