Hotspot

trnV-UAC-ndhC

LSC · 10475-11000 · 526 bp

Back to Crotalaria

Pi 0.0444

MarkerSeek score 71.70

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 526
variable_sites: 36
pi: 0.0444
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	CCGAGAAAGTCTACGGTTCG	AGAGAAGCGGCCATAAACTG	55.0	50.0	58.1	58.0	1022-1099 bp	1.0	86.6
trnV-UAC-ndhC_p2	CCGAGAAAGTCTACGGTTCG	TTCATAGTTACTGTGCCCGC	55.0	50.0	58.1	58.0	1158-1235 bp	1.0	86.4
trnV-UAC-ndhC_p3	AGCCTGACAAAAGGTGGTTT	AGAGAAGCGGCCATAAACTG	45.0	50.0	57.8	58.0	1499-1576 bp	1.0	85.9
trnV-UAC-ndhC_p4	AGCCTGACAAAAGGTGGTTT	TTCATAGTTACTGTGCCCGC	45.0	50.0	57.8	58.0	1635-1712 bp	1.0	85.8
trnV-UAC-ndhC_p5	CCGAGAAAGTCTACGGTTCG	TTTGGGTTGTTCGGTCAAGT	55.0	45.0	58.1	57.9	950-1027 bp	0.75	75.3