Hotspot

rps3

LSC · 81163-81816 · 654 bp

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Pi 0.00612

MarkerSeek score 50.70

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 654
variable_sites: 6
pi: 0.00612
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGACAAGACAAATAAAGGTCCAC	CCACCTGTCATATAACAATTGT	39.1	36.4	57.0	54.2	772-800 bp	1.0	43.3
rps3_p2	GACAAGACAAATAAAGGTCCACT	CCACCTGTCATATAACAATTGT	39.1	36.4	56.7	54.2	771-799 bp	1.0	43.3
rps3_p3	TGACAAGACAAATAAAGGTCCACT	CCACCTGTCATATAACAATTGT	37.5	36.4	58.3	54.2	772-800 bp	1.0	43.3
rps3_p4	TGACAAGACAAATAAAGGTCCA	CCACCTGTCATATAACAATTGT	36.4	36.4	55.6	54.2	772-800 bp	1.0	43.3
rps3_p5	ACAAGACAAATAAAGGTCCACT	CCACCTGTCATATAACAATTGT	36.4	36.4	55.6	54.2	770-798 bp	1.0	43.3