Hotspot

ndhD

SSC · 120418-121923 · 1506 bp

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Pi 0.0062

MarkerSeek score 48.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 14
pi: 0.0062
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGACGGGTGTAAAGCTAAG	GCATTCTTTCCGGAGCAGTA	55.0	50.0	58.0	58.0	2244-2278 bp	1.0	80.6
ndhD_p2	ATACCTTGGGACGGGTGTAA	GCATTCTTTCCGGAGCAGTA	50.0	50.0	58.0	58.0	2251-2285 bp	1.0	80.4
ndhD_p3	GGGACGGGTGTAAAGCTAAG	TTTCTTTGGCGAGCTCCAAT	55.0	45.0	58.0	58.1	2402-2436 bp	1.0	80.1
ndhD_p4	GACGGGTGTAAAGCTAAGCA	GCATTCTTTCCGGAGCAGTA	50.0	50.0	57.9	58.0	2242-2276 bp	1.0	80.1
ndhD_p5	ATACCTTGGGACGGGTGTAA	TTTCTTTGGCGAGCTCCAAT	50.0	45.0	58.0	58.1	2409-2443 bp	1.0	79.9