Hotspot

rpl32-ndhF

SSC · 124015-124972 · 958 bp

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Pi 0.025

MarkerSeek score 64.90

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 958
variable_sites: 32
pi: 0.025
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CTTGCTTGTTTGTCGCACAA	CAAGAGGTCGTGTGAACCAA	45.0	50.0	58.1	58.1	1549-1662 bp	1.0	84.7
rpl32-ndhF_p2	CTTGCTTGTTTGTCGCACAA	AGGGGAAATTGTGCGGATTT	45.0	45.0	58.1	58.1	1775-1888 bp	1.0	84.4
rpl32-ndhF_p3	CGCGGTAGGGTTCAGTTTTA	CAAGAGGTCGTGTGAACCAA	50.0	50.0	57.9	58.1	2081-2180 bp	0.666667	69.9
rpl32-ndhF_p4	CGCGGTAGGGTTCAGTTTTA	AAGGGGAAATTGTGCGGATT	50.0	45.0	57.9	58.1	2308-2407 bp	0.666667	69.4
rpl32-ndhF_p5	CGCGGTAGGGTTCAGTTTTA	AGGGGAAATTGTGCGGATTT	50.0	45.0	57.9	58.1	2307-2406 bp	0.666667	69.4