Hotspot

rpl16-rps3

LSC · 80994-81162 · 169 bp

Back to Croomia

Pi 0.0377

MarkerSeek score 64.60

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 9
pi: 0.0377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	TTTGGATATTTGTAGACGAGT	45.0	33.3	57.1	52.3	211-213 bp	1.0	44.2
rpl16-rps3_p2	ACGAGTCACACACTAAGCAT	TTTGGATATTTGTAGACGAGT	45.0	33.3	56.9	52.3	210-212 bp	1.0	44.2
rpl16-rps3_p3	AACGAGTCACACACTAAGCAT	TTTGGATATTTGTAGACGAGT	42.9	33.3	57.6	52.3	211-213 bp	1.0	44.2
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	ATTTGGATATTTGTAGACGAGT	45.0	31.8	57.1	53.0	212-214 bp	1.0	44.1
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	AATTTGGATATTTGTAGACGAGT	45.0	30.4	57.1	53.8	213-215 bp	1.0	44.1