Hotspot

atpH-atpI

LSC · 13295-13855 · 561 bp

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Pi 0.0126

MarkerSeek score 67.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 10
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCGATAGAAGCAAGCCCTA	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	679-686 bp	1.0	77.7
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	640-647 bp	1.0	77.6
atpH-atpI_p3	TCCGATAGAAGCAAGCCCTA	TAGGTGAATCCATGGAGGGT	50.0	50.0	57.9	57.4	652-659 bp	1.0	77.2
atpH-atpI_p4	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	725-732 bp	1.0	77.1
atpH-atpI_p5	GATAGAAGCAAGCCCTACGG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.8	58.5	676-683 bp	1.0	77.1