Hotspot

trnS-GCU-trnG-UCC

LSC · 7984-8802 · 819 bp

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Pi 0.022

MarkerSeek score 68.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 26
pi: 0.022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	884-918 bp	1.0	56.8
trnS-GCU-trnG-UCC_p2	TCGAACCCTCGGTACCAATA	ACGAATCACACTTTTACCACT	50.0	38.1	57.9	55.5	917-951 bp	1.0	55.8
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	887-921 bp	1.0	55.8
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	886-920 bp	1.0	55.8
trnS-GCU-trnG-UCC_p5	GTCTTAAGCGATCGAAGGGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	953-987 bp	1.0	55.6