Hotspot

accD

LSC · 54712-56106 · 1395 bp

Back to Crocus

Pi 0.0119
MarkerSeek score 51.10
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1395
variable_sites
25
pi
0.0119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TCAAATAAGGAGCAAGAAGACTCT ACCTGTCCAATACAATTCGCT 37.5 42.9 57.6 57.6 1463-1467 bp 1.0 41.7
accD_p2 TCAAATAAGGAGCAAGAAGACTC ACCTGTCCAATACAATTCGCT 39.1 42.9 56.3 57.6 1463-1467 bp 1.0 41.7
accD_p3 CAAATAAGGAGCAAGAAGACTCT ACCTGTCCAATACAATTCGCT 39.1 42.9 56.3 57.6 1462-1466 bp 1.0 41.7
accD_p4 TCAAATAAGGAGCAAGAAGACTCT ACCTGTCCAATACAATTCGC 37.5 45.0 57.6 56.1 1463-1467 bp 1.0 41.7
accD_p5 TCAAATAAGGAGCAAGAAGACT ACCTGTCCAATACAATTCGCT 36.4 42.9 55.1 57.6 1463-1467 bp 1.0 41.7