Hotspot

rps12-rps7

IRa · 137150-137207 · 58 bp

Back to Crocus

Pi 0.023
MarkerSeek score 54.70
Primer available yes
Label rps12-rps7

Feature Metadata

label_name
rps12-rps7
feature_type
igs
region
IRa
length_bp
58
variable_sites
2
pi
0.023
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-rps7_p1 CTGTGGAAAGCCGTATTCGA TTCTTCTGCAGTACCTCGAC 50.0 50.0 58.0 57.0 190 bp 1.0 72.7
rps12-rps7_p2 TCTGTGGAAAGCCGTATTCG TTCTTCTGCAGTACCTCGAC 50.0 50.0 58.0 57.0 191 bp 1.0 72.7
rps12-rps7_p3 CTGTGGAAAGCCGTATTCGA TCTTCTGCAGTACCTCGACG 50.0 55.0 58.0 59.2 189 bp 1.0 71.2
rps12-rps7_p4 TCTGTGGAAAGCCGTATTCG TCTTCTGCAGTACCTCGACG 50.0 55.0 58.0 59.2 190 bp 1.0 71.2
rps12-rps7_p5 CTGTGGAAAGCCGTATTCGA TTTCTTCTGCAGTACCTCGAC 50.0 47.6 58.0 57.7 191 bp 1.0 70.4