Hotspot

rpl32-trnL

SSC · 110327-111019 · 693 bp

Back to Crocus

Pi 0.0135
MarkerSeek score 41.60
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
SSC
length_bp
693
variable_sites
14
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 AAACGCAGCACTTTGAACTT AGCGTGTCTACCGATTTCAC 40.0 50.0 57.1 58.0 1174-1207 bp 1.0 74.9
rpl32-trnL_p2 AAACGCAGCACTTTGAACTT CAGCGTGTCTACCGATTTCA 40.0 50.0 57.1 58.0 1175-1208 bp 1.0 74.8
rpl32-trnL_p3 AAACGCAGCACTTTGAACTT TGGCGAAACCTTGGGTAAAA 40.0 45.0 57.1 57.9 1651-1684 bp 1.0 73.9
rpl32-trnL_p4 AAACGCAGCACTTTGAACTT TCGAAGAAATCCAACGGGTC 40.0 50.0 57.1 57.9 1501-1534 bp 1.0 73.8
rpl32-trnL_p5 TTTTTGTGCGACAAACAGGT AGCGTGTCTACCGATTTCAC 40.0 50.0 57.0 58.0 760 bp 0.666667 58.4