Hotspot

ndhF-rpl32

SSC · 109586-110152 · 567 bp

Back to Crocus

Pi 0.0165
MarkerSeek score 49.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
567
variable_sites
14
pi
0.0165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAGCAGAAGTCCAAATCCCA TGCAGCAAAATATCCCTTTTTCT 45.0 34.8 57.0 57.3 735-976 bp 1.0 44.3
ndhF-rpl32_p2 AGCAGAAGTCCAAATCCCAT TGCAGCAAAATATCCCTTTTTCT 45.0 34.8 56.8 57.3 734-975 bp 1.0 42.4
ndhF-rpl32_p3 GTCGGAATAAGCAGAAGTCCA TGCAGCAAAATATCCCTTTTTCT 47.6 34.8 57.8 57.3 743-984 bp 1.0 42.1
ndhF-rpl32_p4 TGTCGGAATAAGCAGAAGTCC TGCAGCAAAATATCCCTTTTTCT 47.6 34.8 57.8 57.3 744-985 bp 1.0 42.1
ndhF-rpl32_p5 ACTGGAAACGGAAGAAAAGGT TGCAGCAAAATATCCCTTTTTCT 42.9 34.8 57.7 57.3 704-945 bp 1.0 42.1