Hotspot

rps7-rps12

IRb · 94922-94979 · 58 bp

Back to Crocus

Pi 0.023
MarkerSeek score 54.70
Primer available yes
Label rps7-rps12

Feature Metadata

label_name
rps7-rps12
feature_type
igs
region
IRb
length_bp
58
variable_sites
2
pi
0.023
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-rps12_p1 TTCTTCTGCAGTACCTCGAC CTGTGGAAAGCCGTATTCGA 50.0 50.0 57.0 58.0 190 bp 1.0 72.7
rps7-rps12_p2 TTCTTCTGCAGTACCTCGAC TCTGTGGAAAGCCGTATTCG 50.0 50.0 57.0 58.0 191 bp 1.0 72.7
rps7-rps12_p3 TCTTCTGCAGTACCTCGACG CTGTGGAAAGCCGTATTCGA 55.0 50.0 59.2 58.0 189 bp 1.0 71.2
rps7-rps12_p4 TCTTCTGCAGTACCTCGACG TCTGTGGAAAGCCGTATTCG 55.0 50.0 59.2 58.0 190 bp 1.0 71.2
rps7-rps12_p5 TTTCTTCTGCAGTACCTCGAC CTGTGGAAAGCCGTATTCGA 47.6 50.0 57.7 58.0 191 bp 1.0 70.4