Hotspot

rpl20-rps12

LSC · 66439-67183 · 745 bp

Back to Crocus

Pi 0.00895
MarkerSeek score 52.60
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
745
variable_sites
10
pi
0.00895
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTTTTGTTCGACGTCTCCGA AAATGTCACGAAATCCCCCG 45.0 50.0 57.8 58.5 858-861 bp 1.0 75.3
rpl20-rps12_p2 TTCGACGTCTCCGAGCTATA AAATGTCACGAAATCCCCCG 50.0 50.0 57.8 58.5 852-855 bp 1.0 75.1
rpl20-rps12_p3 TTTTGTTCGACGTCTCCGAG AAATGTCACGAAATCCCCCG 50.0 50.0 58.2 58.5 857-860 bp 1.0 75.1
rpl20-rps12_p4 TTTTTGTTCGACGTCTCCGA CAGCGTCGAGGAACATGTAC 45.0 55.0 57.8 58.7 821-824 bp 1.0 73.9
rpl20-rps12_p5 TTCGACGTCTCCGAGCTATA CAGCGTCGAGGAACATGTAC 50.0 55.0 57.8 58.7 815-818 bp 1.0 73.7