Hotspot

trnG-UCC-trnfM-CAU

LSC · 34719-34847 · 129 bp

Back to Crocus

Pi 0.031
MarkerSeek score 55.80
Primer available yes
Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name
trnG-UCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
129
variable_sites
6
pi
0.031
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 195-197 bp 1.0 84.1
trnG-UCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 196-198 bp 1.0 84.1
trnG-UCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 189-191 bp 1.0 83.7
trnG-UCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 190-192 bp 1.0 83.7
trnG-UCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC TCACGGGTTCAAATCCTGTC 50.0 50.0 58.1 57.8 188-190 bp 1.0 83.2