Hotspot

trnR-UCU-atpA

LSC · 9115-9168 · 54 bp

Back to Crocus

Pi 0.0494
MarkerSeek score 69.30
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
54
variable_sites
4
pi
0.0494
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC ATTTACCGAGCAAGCCGAAA 47.6 45.0 57.3 58.2 153-158 bp 1.0 69.6
trnR-UCU-atpA_p2 GGAATGAAAAGCGTCCATTGT ATTTACCGAGCAAGCCGAAA 42.9 45.0 57.4 58.2 213-218 bp 1.0 69.2
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC AGACATTTACCGAGCAAGCC 47.6 50.0 57.3 58.3 157-162 bp 1.0 68.9
trnR-UCU-atpA_p4 GGAATGAAAAGCGTCCATTGT AGACATTTACCGAGCAAGCC 42.9 50.0 57.4 58.3 217-222 bp 1.0 68.6
trnR-UCU-atpA_p5 AGGAATGAAAAGCGTCCATTG ATTTACCGAGCAAGCCGAAA 42.9 45.0 57.1 58.2 214-219 bp 1.0 67.0