Hotspot

rps16-trnQ-UUG

LSC · 5849-6434 · 586 bp

Back to Crocus

Pi 0.0148
MarkerSeek score 46.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
586
variable_sites
13
pi
0.0148
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 652-659 bp 1.0 81.0
rps16-trnQ-UUG_p2 TGCTTTCTACCGCATCGTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 641-648 bp 1.0 80.5
rps16-trnQ-UUG_p3 TTGCTTTCTACCGCATCGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 642-649 bp 1.0 80.5
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 663-670 bp 1.0 80.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 656-663 bp 1.0 79.9