Hotspot

rps15

SSC · 126896-127165 · 270 bp

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Pi 0
MarkerSeek score 19.70
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
270
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
19.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 AACACCATGCATTGATGGGT ATCTCCCAGACGTCGTAGAG 45.0 55.0 58.1 58.0 1185-1190 bp 1.0 79.9
rps15_p2 GGTAGATCAGCAGCAACTCC ATCTCCCAGACGTCGTAGAG 55.0 55.0 58.1 58.0 1613-1618 bp 1.0 79.8
rps15_p3 GTAGATCAGCAGCAACTCCC ATCTCCCAGACGTCGTAGAG 55.0 55.0 58.1 58.0 1612-1617 bp 1.0 79.8
rps15_p4 CTTTCCAAACGTTCTGGTGC ATCTCCCAGACGTCGTAGAG 50.0 55.0 57.9 58.0 1382-1387 bp 1.0 79.3
rps15_p5 GAGTCTGTGCACCGATATCC ATCTCCCAGACGTCGTAGAG 55.0 55.0 57.9 58.0 1498-1503 bp 1.0 79.1