Hotspot

ndhD

SSC · 118924-120425 · 1502 bp

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Pi 0.000888
MarkerSeek score 34.30
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1502
variable_sites
2
pi
0.000888
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 ACTTTGCTTTCTGTCTCATT CAAAAGCCCGTACTCGAGAA 35.0 50.0 53.0 57.9 1608-1609 bp 1.0 40.1
ndhD_p2 ACTTTGCTTTCTGTCTCATT CCTTTTTACCGGCAAAAGCC 35.0 50.0 53.0 58.2 1620-1621 bp 1.0 40.1
ndhD_p3 ACTTTGCTTTCTGTCTCATT CTTTTTACCGGCAAAAGCCC 35.0 50.0 53.0 58.2 1619-1620 bp 1.0 40.1
ndhD_p4 ACTTTGCTTTCTGTCTCATT TCCTTTTTACCGGCAAAAGC 35.0 45.0 53.0 57.2 1621-1622 bp 1.0 40.1
ndhD_p5 ACTTTGCTTTCTGTCTCATT AAAGCCCGTACTCGAGAAAA 35.0 45.0 53.0 57.2 1606-1607 bp 1.0 40.1