Hotspot

clpP

LSC · 72567-74633 · 2067 bp

Back to Crinum

Pi 0.00274
MarkerSeek score 59.40
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2067
variable_sites
8
pi
0.00274
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TGGGTTGGTTTAGATCGATCT AACTCATTCTTACGTTTCCA 42.9 35.0 56.4 52.5 2238-2239 bp 1.0 40.4
clpP_p2 TGGGTTGGTTTAGATCGATCT AAACTCATTCTTACGTTTCCA 42.9 33.3 56.4 53.4 2239-2240 bp 1.0 40.4
clpP_p3 TGGGTTGGTTTAGATCGATCT ACTCATTCTTACGTTTCCAT 42.9 35.0 56.4 52.2 2237-2238 bp 1.0 40.4
clpP_p4 TGGGTTGGTTTAGATCGATCTT AACTCATTCTTACGTTTCCA 40.9 35.0 57.2 52.5 2238-2239 bp 1.0 40.4
clpP_p5 TGGGTTGGTTTAGATCGATCT AACTCATTCTTACGTTTCCAT 42.9 33.3 56.4 53.2 2238-2239 bp 1.0 40.4