Hotspot

atpF

LSC · 12253-13685 · 1433 bp

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Pi 0.000934
MarkerSeek score 63.60
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1433
variable_sites
2
pi
0.000934
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 ACTAATTTCGTCAGCTCGAA GGCGAGCCAAGTGATACAAA 40.0 50.0 54.9 58.6 1570-1605 bp 1.0 53.3
atpF_p2 ACTAATTTCGTCAGCTCGAA ATCGATCAAAAAGGGCGAGC 40.0 50.0 54.9 59.0 1583-1618 bp 1.0 49.8
atpF_p3 ACTAATTTCGTCAGCTCGAA ATCAAAAAGGGCGAGCCAA 40.0 47.4 54.9 57.9 1579-1614 bp 1.0 49.3
atpF_p4 ACTAATTTCGTCAGCTCGAA ATCAAAAAGGGCGAGCCAAG 40.0 50.0 54.9 59.1 1579-1614 bp 1.0 48.8
atpF_p5 ACTAATTTCGTCAGCTCGAA GATCAAAAAGGGCGAGCCAA 40.0 50.0 54.9 59.1 1580-1615 bp 1.0 48.8