Hotspot

psaC-ndhE

SSC · 120792-121290 · 499 bp

Back to Crinum

Pi 0.00601
MarkerSeek score 62.90
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
499
variable_sites
4
pi
0.00601
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA GGAAGCAGCTATTGGGCTAG 50.0 55.0 58.1 58.1 692-711 bp 1.0 79.8
psaC-ndhE_p2 CCAATACATCTGTGGGGCAA GGAAGCAGCTATTGGGCTAG 50.0 55.0 57.9 58.1 669-688 bp 1.0 79.7
psaC-ndhE_p3 AATACATCTGTGGGGCAAGC GGAAGCAGCTATTGGGCTAG 50.0 55.0 58.2 58.1 667-686 bp 1.0 79.0
psaC-ndhE_p4 AGCTTTACATCCGTCCCAAG GGAAGCAGCTATTGGGCTAG 50.0 55.0 57.6 58.1 698-717 bp 1.0 77.6
psaC-ndhE_p5 TTCCAATACATCTGTGGGGC GGAAGCAGCTATTGGGCTAG 50.0 55.0 57.6 58.1 671-690 bp 1.0 77.4