Hotspot

ccsA-ndhD

SSC · 118666-118923 · 258 bp

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Pi 0.00919
MarkerSeek score 60.40
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
258
variable_sites
3
pi
0.00919
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGGCACTTGTAGCTTCTACA CGGATTTCGTTCTCTCGCTA 50.0 50.0 57.6 57.5 434-439 bp 1.0 75.0
ccsA-ndhD_p2 CGGCACTTGTAGCTTCTACA ATCCGGATTTCGTTCTCTCG 50.0 50.0 57.6 57.5 437-442 bp 1.0 74.8
ccsA-ndhD_p3 CGAATTCGGCACTTGTAGCT CGGATTTCGTTCTCTCGCTA 50.0 50.0 58.6 57.5 440-445 bp 1.0 72.7
ccsA-ndhD_p4 TCGGCACTTGTAGCTTCTAC CGGATTTCGTTCTCTCGCTA 50.0 50.0 57.4 57.5 435-440 bp 1.0 72.7
ccsA-ndhD_p5 CGAATTCGGCACTTGTAGCT ATCCGGATTTCGTTCTCTCG 50.0 50.0 58.6 57.5 443-448 bp 1.0 72.5