Hotspot

psbE-petL

LSC · 67480-68486 · 1007 bp

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Pi 0.00133
MarkerSeek score 62.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1007
variable_sites
2
pi
0.00133
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AGCAGCCAATAGAAAACCGA 50.0 45.0 57.9 57.5 1134-1153 bp 1.0 77.5
psbE-petL_p2 ACTGACGAATAACCAACCCG AGCAGCCAATAGAAAACCGA 50.0 45.0 57.9 57.5 1147-1166 bp 1.0 77.2
psbE-petL_p3 ACGAATAACCAACCCGCAAT AGCAGCCAATAGAAAACCGA 45.0 45.0 58.2 57.5 1143-1162 bp 1.0 76.5
psbE-petL_p4 CGAATAACCAACCCGCAATG AGCAGCCAATAGAAAACCGA 50.0 45.0 57.8 57.5 1142-1161 bp 1.0 76.4
psbE-petL_p5 ACCAACCCGCAATGAATAGG AGCAGCCAATAGAAAACCGA 50.0 45.0 58.2 57.5 1136-1155 bp 1.0 76.0