Hotspot

ycf4-cemA

LSC · 62781-63680 · 900 bp

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Pi 0.00148
MarkerSeek score 60.60
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
900
variable_sites
2
pi
0.00148
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA ATGAGAGAGAGACCCACCAG 45.0 55.0 57.9 57.8 1005-1011 bp 1.0 78.8
ycf4-cemA_p2 CTTGCGCGTACCAATTGAAG ATGAGAGAGAGACCCACCAG 50.0 55.0 58.1 57.8 1007-1013 bp 1.0 78.5
ycf4-cemA_p3 TGCGCGTACCAATTGAAGTA GGAGGGAAGTCGATGCTTTC 45.0 55.0 57.9 58.3 957-963 bp 1.0 77.4
ycf4-cemA_p4 GGAACCATTCCCTTGACTCG ATGAGAGAGAGACCCACCAG 55.0 55.0 58.3 57.8 1087-1093 bp 1.0 77.3
ycf4-cemA_p5 CTTGCGCGTACCAATTGAAG GGAGGGAAGTCGATGCTTTC 50.0 55.0 58.1 58.3 959-965 bp 1.0 77.1