Hotspot

ndhC-trnV-UAC

LSC · 52028-53758 · 1731 bp

Back to Crinum

Pi 0.00347
MarkerSeek score 61.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1731
variable_sites
9
pi
0.00347
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1854-1882 bp 1.0 78.6
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 1936-1964 bp 1.0 76.3
ndhC-trnV-UAC_p3 TCCTTCACTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1867-1895 bp 1.0 76.0
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1853-1881 bp 1.0 75.8
ndhC-trnV-UAC_p5 CTGGTCCTTCACTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.4 57.8 1871-1899 bp 1.0 74.9