Hotspot

trnF-GAA-ndhJ

LSC · 49631-50345 · 715 bp

Back to Crinum

Pi 0.000932
MarkerSeek score 62.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
715
variable_sites
1
pi
0.000932
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 807-825 bp 1.0 80.4
trnF-GAA-ndhJ_p2 GGAAATCGTCGGGATAGCTC TGAAAGTTGGATAGGCTGGC 55.0 50.0 57.9 57.9 853-871 bp 1.0 80.2
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 797-815 bp 1.0 79.8
trnF-GAA-ndhJ_p4 GGAAATCGTCGGGATAGCTC ATAGGCTGGCCCTTACGTAA 55.0 50.0 57.9 58.2 843-861 bp 1.0 79.6
trnF-GAA-ndhJ_p5 ATCGTCGGGATAGCTCAGTT TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.3 57.9 849-867 bp 1.0 78.3