Hotspot

rps16-trnQ-UUG

LSC · 5813-7310 · 1498 bp

Back to Crinum

Pi 0.00401
MarkerSeek score 63.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1498
variable_sites
9
pi
0.00401
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCGAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1566-1594 bp 1.0 81.5
rps16-trnQ-UUG_p2 ATCCACAGCGAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1570-1598 bp 1.0 80.5
rps16-trnQ-UUG_p3 CCACAGCGAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 1564-1592 bp 1.0 79.8
rps16-trnQ-UUG_p4 ACACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.7 58.1 1538-1566 bp 1.0 79.4
rps16-trnQ-UUG_p5 CCACAGCGAATCATGTCCTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.3 57.8 1568-1596 bp 1.0 78.7