Hotspot

petB

LSC · 73829-74889 · 1061 bp

Back to Crinipes

Pi 0.00378

MarkerSeek score 61.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1061
variable_sites: 4
pi: 0.00378
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGAGACTCAATCATCGTGGC	ACCACAACCTACCCGATATGA	50.0	47.6	57.7	58.2	1140-1144 bp	1.0	68.2
petB_p2	TGAGACTCAATCATCGTGGC	ACCACAACCTACCCGATATG	50.0	50.0	57.7	56.7	1140-1144 bp	1.0	67.8
petB_p3	TCGAAGAACCCTTGCCTTTT	ACCACAACCTACCCGATATGA	45.0	47.6	57.6	58.2	1168-1172 bp	1.0	67.7
petB_p4	TTCGAAGAACCCTTGCCTTT	ACCACAACCTACCCGATATGA	45.0	47.6	57.6	58.2	1169-1173 bp	1.0	67.7
petB_p5	TGAGACTCAATCATCGTGGC	TGAAATACCACAACCTACCCG	50.0	47.6	57.7	57.7	1146-1150 bp	1.0	67.5