Hotspot

ccsA

SSC · 107926-108894 · 969 bp

Back to Crinipes

Pi 0.00619

MarkerSeek score 48.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 6
pi: 0.00619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ACTCTCCCAATCTCGACGAT	TCTGGACCACGAGAACTCTT	50.0	50.0	57.9	58.0	1521 bp	1.0	80.1
ccsA_p2	ACTCTCCCAATCTCGACGAT	TTCTGGACCACGAGAACTCT	50.0	50.0	57.9	58.0	1522 bp	1.0	80.1
ccsA_p3	ACTCTCCCAATCTCGACGAT	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	1525 bp	1.0	80.0
ccsA_p4	TAGACACGCTGCTCTTAGGA	TCTGGACCACGAGAACTCTT	50.0	50.0	57.9	58.0	1432 bp	1.0	79.6
ccsA_p5	TAGACACGCTGCTCTTAGGA	TTCTGGACCACGAGAACTCT	50.0	50.0	57.9	58.0	1433 bp	1.0	79.6