Hotspot

ndhF-rpl32

SSC · 106188-107084 · 897 bp

Back to Crinipes

Pi 0.019

MarkerSeek score 65.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 897
variable_sites: 17
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGTCCAAACCCCATTGACA	TGACATCGAAGTACGTTTTT	45.0	35.0	57.2	53.2	1008-1042 bp	1.0	41.3
ndhF-rpl32_p2	AAGTCCAAACCCCATTGACA	TTGACATCGAAGTACGTTTT	45.0	35.0	57.2	53.2	1009-1043 bp	1.0	41.3
ndhF-rpl32_p3	AAGTCCAAACCCCATTGACA	TTTGACATCGAAGTACGTTT	45.0	35.0	57.2	53.2	1010-1044 bp	1.0	41.3
ndhF-rpl32_p4	AAGTCCAAACCCCATTGACA	TTTTGACATCGAAGTACGTT	45.0	35.0	57.2	53.2	1011-1045 bp	1.0	41.3
ndhF-rpl32_p5	AAGTCCAAACCCCATTGACA	TTTTTGACATCGAAGTACGT	45.0	35.0	57.2	53.2	1012-1046 bp	1.0	41.3