Hotspot

ycf4-cemA

LSC · 60267-60645 · 379 bp

Back to Crinipes

Pi 0.0132

MarkerSeek score 61.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 5
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.9	57.8	483 bp	1.0	78.2
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	GAAAGAGACTCCCCAGGGTA	45.0	55.0	57.9	57.8	480 bp	1.0	78.2
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.1	57.8	485 bp	1.0	77.9
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	GAAAGAGACTCCCCAGGGTA	50.0	55.0	58.1	57.8	482 bp	1.0	77.9
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	512 bp	1.0	77.6