Hotspot

trnD-GUC-psbM

LSC · 17423-18470 · 1048 bp

Back to Crinipes

Pi 0.0086
MarkerSeek score 63.80
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1048
variable_sites
9
pi
0.0086
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 CCTCGCAGGGATTGTAGTTC TCATTTTGGCTGGCTGTTTT 55.0 40.0 58.1 56.7 1230 bp 1.0 69.8
trnD-GUC-psbM_p2 TACACCTCGCAGGGATTGTA TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.1 56.7 1234 bp 1.0 69.1
trnD-GUC-psbM_p3 CCTCGCAGGGATTGTAGTTC AATCATTTTGGCTGGCTGTT 55.0 40.0 58.1 56.5 1232 bp 1.0 67.9
trnD-GUC-psbM_p4 CCTCGCAGGGATTGTAGTTC ATCATTTTGGCTGGCTGTTT 55.0 40.0 58.1 56.5 1231 bp 1.0 67.9
trnD-GUC-psbM_p5 CCTCGCAGGGATTGTAGTTC TCATTTTGGCTGGCTGTTTTT 55.0 38.1 58.1 57.4 1230 bp 1.0 67.7