Hotspot

rpl32-trnL-UAG

SSC · 108754-109602 · 849 bp

Back to Crepis

Pi 0.0684
MarkerSeek score 80.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
849
variable_sites
151
pi
0.0684
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 651-1045 bp 1.0 88.8
rpl32-trnL-UAG_p2 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 652-1046 bp 1.0 88.8
rpl32-trnL-UAG_p3 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 683-1062 bp 0.875 84.0
rpl32-trnL-UAG_p4 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 684-1063 bp 0.875 84.0
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG GAAGTAGCAAAGCCTTGGGT 50.0 50.0 57.6 58.1 1191-1584 bp 0.875 83.4