Hotspot

psbE-petL

LSC · 61221-62374 · 1154 bp

Back to Crepis

Pi 0.0414
MarkerSeek score 82.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1154
variable_sites
136
pi
0.0414
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAATAACTAGTTATGGTAGGCA 50.0 31.8 57.9 52.3 1281-1377 bp 1.0 52.0
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AAATAACTAGTTATGGTAGGCA 50.0 31.8 57.8 52.3 1204-1300 bp 1.0 52.0
psbE-petL_p3 ACGAATAACCAACCCGCAAT AAATAACTAGTTATGGTAGGCA 45.0 31.8 58.2 52.3 1290-1386 bp 1.0 52.0
psbE-petL_p4 TGCTGACGAATAACCAACCC AAATAACTAGTTATGGTAGGCA 50.0 31.8 58.2 52.3 1295-1391 bp 1.0 52.0
psbE-petL_p5 CGAATAACCAACCCGCAATG AAATAACTAGTTATGGTAGGCA 50.0 31.8 57.8 52.3 1289-1385 bp 1.0 52.0