Hotspot

petA-psbJ

LSC · 59653-60429 · 777 bp

Back to Crepis

Pi 0.0453
MarkerSeek score 81.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
777
variable_sites
101
pi
0.0453
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTGACGGGAGCAAGAGTAAC TGGAGGGAAGTGGGGTAAAT 55.0 50.0 58.0 57.9 1225-1297 bp 1.0 90.7
petA-psbJ_p2 CTGACGGGAGCAAGAGTAAC TCCCTATTCATTGCGGGTTG 55.0 50.0 58.0 57.9 1791-1863 bp 1.0 89.1
petA-psbJ_p3 CGGATTTGGTCAGGGAGATG TCCCTATTCATTGCGGGTTG 55.0 50.0 58.0 57.9 1571-1643 bp 1.0 89.1
petA-psbJ_p4 GCATCTGATGGACGTCAAGT TGGAGGGAAGTGGGGTAAAT 50.0 50.0 58.0 57.9 1118-1190 bp 0.75 79.4
petA-psbJ_p5 GCATCTGATGGACGTCAAGT TCCCTATTCATTGCGGGTTG 50.0 50.0 58.0 57.9 1684-1756 bp 0.75 78.0