Hotspot

ndhC-trnV-UAC

LSC · 49000-49966 · 967 bp

Back to Crepis

Pi 0.0525
MarkerSeek score 82.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
967
variable_sites
132
pi
0.0525
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTTGCTACTCGGAGCTA ATGAAAGAGCCCAATGCACA 50.0 45.0 57.9 58.1 529-1411 bp 1.0 90.3
ndhC-trnV-UAC_p2 GCGTCGCCTATTGGTTCTAT ATGAAAGAGCCCAATGCACA 50.0 45.0 57.8 58.1 579-1461 bp 1.0 90.2
ndhC-trnV-UAC_p3 GTCTAGGACTCGACCTTGGT ATGAAAGAGCCCAATGCACA 55.0 45.0 58.2 58.1 1043-1925 bp 1.0 89.1
ndhC-trnV-UAC_p4 CGTGAGCCTATTAGGGAAGC ATGAAAGAGCCCAATGCACA 55.0 45.0 57.8 58.1 1668-1878 bp 0.875 83.1
ndhC-trnV-UAC_p5 TCCTTTGCTACTCGGAGCTA GACAAGGGTTCCATCCGTTT 50.0 50.0 57.9 58.1 1579-1790 bp 0.5 63.9