Hotspot

petN-psbM

LSC · 10312-10807 · 496 bp

Back to Crepis

Pi 0.0531
MarkerSeek score 82.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
496
variable_sites
73
pi
0.0531
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GCGGCTAACTAGTTGTGGAA TTTGATTGGTCCGGGAATCC 50.0 50.0 57.9 57.9 1460-1516 bp 0.625 71.1
petN-psbM_p2 TCCGTTCTAGCGTCATTGTG GTCCCGACGGATCCAATAAC 50.0 55.0 58.0 58.1 1502-1519 bp 0.375 58.5
petN-psbM_p3 TCCGTTCTAGCGTCATTGTG TTTGATTGGTCCGGGAATCC 50.0 50.0 58.0 57.9 1138-1152 bp 0.375 57.9
petN-psbM_p4 TCCGTTCTAGCGTCATTGTG AGTCCCGACGGATCCAATAA 50.0 50.0 58.0 58.2 1503-1520 bp 0.375 57.9
petN-psbM_p5 TCCGTTCTAGCGTCATTGTG CCGCGACTCATCTTTCTCAT 50.0 50.0 58.0 57.8 1356-1370 bp 0.25 50.1