Hotspot

rps16-trnQ-UUG

LSC · 6298-7242 · 945 bp

Back to Crepis

Pi 0.0568
MarkerSeek score 85.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
945
variable_sites
162
pi
0.0568
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GATCGTTGCCCCTGAGAAAT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1747-1832 bp 1.0 91.2
rps16-trnQ-UUG_p2 GATCGTTGCCCCTGAGAAAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1163-1247 bp 1.0 91.0
rps16-trnQ-UUG_p3 ATACCGACGGTTGATTCGTG CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 1911-1996 bp 1.0 90.9
rps16-trnQ-UUG_p4 AAGCGGTGTTTCCTTGTTCT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.9 58.1 1511-1546 bp 0.875 83.8
rps16-trnQ-UUG_p5 AAGCGGTGTTTCCTTGTTCT CTTACAGCAGCTTGCCAAAC 45.0 50.0 57.9 57.9 2093-2131 bp 0.875 83.1