Hotspot

trnK-UUU-rps16

LSC · 4382-5161 · 780 bp

Back to Crepis

Pi 0.0468
MarkerSeek score 83.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
780
variable_sites
103
pi
0.0468
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1190-1233 bp 1.0 91.1
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1189-1232 bp 1.0 91.1
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG ACGAATCAACCGTCGGTATG 55.0 50.0 58.0 58.1 1627-1675 bp 1.0 90.8
trnK-UUU-rps16_p4 CCCTTTCAGGATCAGTCGTG AGAACAAGGAAACACCGCTT 55.0 45.0 58.0 57.9 1494-1542 bp 0.875 84.6
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG AGAACAAGGAAACACCGCTT 55.0 45.0 58.0 57.9 1493-1541 bp 0.875 84.6