Hotspot

ycf2

Genome · 1-5361 · 5361 bp

Back to Crepidomanes

Pi 0.17

MarkerSeek score 74.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 5361
variable_sites: 894
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TTGAGAGAGCTCGAATTGGC	ACAGGATCGCAGTCTGTTTC	50.0	50.0	58.0	57.9	888 bp	0.5	59.2
ycf2_p2	AAGCCCTGATGCTATGCTTC	ACAGGATCGCAGTCTGTTTC	50.0	50.0	58.0	57.9	583 bp	0.5	59.1
ycf2_p3	TTGAGAGAGCTCGAATTGGC	TTGAATCGCTACAGGATCGC	50.0	50.0	58.0	58.1	898 bp	0.5	58.7
ycf2_p4	AAGCCCTGATGCTATGCTTC	TTGAATCGCTACAGGATCGC	50.0	50.0	58.0	58.1	593 bp	0.5	58.7
ycf2_p5	GTTCGCAGCGATACACTGTA	ACAGGATCGCAGTCTGTTTC	50.0	50.0	58.1	57.9	515 bp	0.5	58.5