Hotspot

rrn16-trnV-GAC

Genome · 138371-138769 · 399 bp

Back to Crepidomanes

Pi 0.193

MarkerSeek score 84.00

Primer available yes

Label rrn16-trnV-GAC

Feature Metadata

label_name: rrn16-trnV-GAC
feature_type: igs
region: Genome
length_bp: 399
variable_sites: 76
pi: 0.193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnV-GAC_p1	CCACTAGAAACACCACGAGG	GGTGCAAGTCATCAGTTCGA	55.0	50.0	57.9	58.2	531-1404 bp	1.0	84.4
rrn16-trnV-GAC_p2	GAGCCAGGATCAAACTCTCC	GGTGCAAGTCATCAGTTCGA	55.0	50.0	57.7	58.2	460-1333 bp	1.0	83.0
rrn16-trnV-GAC_p3	AGCCAGGATCAAACTCTCCA	GGTGCAAGTCATCAGTTCGA	50.0	50.0	58.3	58.2	459-1332 bp	1.0	82.9
rrn16-trnV-GAC_p4	CCACTAGAAACACCACGAGG	AACTCAGCGGTAGAGTGTCA	55.0	50.0	57.9	58.4	560-1433 bp	1.0	82.8
rrn16-trnV-GAC_p5	CCACTAGAAACACCACGAGG	TGGTGCAAGTCATCAGTTCG	55.0	50.0	57.9	58.5	532-1405 bp	1.0	82.1