Hotspot

trnN-GUU-trnR-ACG

Genome · 130314-130747 · 434 bp

Back to Crepidomanes

Pi 0.186

MarkerSeek score 89.90

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 434
variable_sites: 77
pi: 0.186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CTCGGTAGCTCAGTGGTAGA	GGATGGAGAGAGGAAGGGTT	55.0	55.0	58.0	58.1	624-763 bp	1.0	89.0
trnN-GUU-trnR-ACG_p2	TCGGTAGCTCAGTGGTAGAG	GGATGGAGAGAGGAAGGGTT	55.0	55.0	58.0	58.1	623-762 bp	1.0	89.0
trnN-GUU-trnR-ACG_p3	GCAATCTTCCTCGGTAGCTC	GGATGGAGAGAGGAAGGGTT	55.0	55.0	58.1	58.1	633-772 bp	1.0	88.1
trnN-GUU-trnR-ACG_p4	CTCGGTAGCTCAGTGGTAGA	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	598-697 bp	1.0	87.7
trnN-GUU-trnR-ACG_p5	TCGGTAGCTCAGTGGTAGAG	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	597-696 bp	1.0	87.7