Hotspot

trnP-GGG-trnL-UAG

Genome · 112022-112240 · 219 bp

Back to Crepidomanes

Pi 0.198

MarkerSeek score 82.80

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 219
variable_sites: 41
pi: 0.198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	CCAAGCTACGCTATATCCCG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	57.7	57.9	278-280 bp	1.0	88.2
trnP-GGG-trnL-UAG_p2	CCAAGCTACGCTATATCCCG	CAGCGTGTCTACCTGTTTCA	55.0	50.0	57.7	57.9	269-271 bp	1.0	88.1
trnP-GGG-trnL-UAG_p3	CCAAGCTACGCTATATCCCG	AGCGTGTCTACCTGTTTCAC	55.0	50.0	57.7	57.8	268-270 bp	1.0	88.1
trnP-GGG-trnL-UAG_p4	CCAAGCTACGCTATATCCCG	TAAGAGCAGCGTGTCTACCT	55.0	50.0	57.7	58.2	275-277 bp	1.0	87.9
trnP-GGG-trnL-UAG_p5	CCAAGCTACGCTATATCCCG	CTAAGAGCAGCGTGTCTACC	55.0	55.0	57.7	57.8	276-278 bp	1.0	87.7