Hotspot

rpl32-trnP-GGG

Genome · 111444-111947 · 504 bp

Back to Crepidomanes

Pi 0.277

MarkerSeek score 78.30

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 504
variable_sites: 114
pi: 0.277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	CCGTTTCACATTGATTGGCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1504 bp	0.5	59.0
rpl32-trnP-GGG_p2	CCGTTTCACATTGATTGGCC	CAGCGTGTCTACCTGTTTCA	50.0	50.0	58.0	57.9	1495 bp	0.5	58.8
rpl32-trnP-GGG_p3	CCGTTTCACATTGATTGGCC	AGCGTGTCTACCTGTTTCAC	50.0	50.0	58.0	57.8	1494 bp	0.5	58.8
rpl32-trnP-GGG_p4	CCGTTTCACATTGATTGGCC	TAAGAGCAGCGTGTCTACCT	50.0	50.0	58.0	58.2	1501 bp	0.5	58.6
rpl32-trnP-GGG_p5	CCGTTTCACATTGATTGGCC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	1502 bp	0.5	58.4